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KMID : 0903519960390060449
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1996 Volume.39 No. 6 p.449 ~ p.454
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Optimal Production of Protease from Entomopathogenic Fungus Beauveria bassiana
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Ko Hwi-Jin
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Abstract
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We investigated the optimal condition for the production of extracellular protease(a cuticle-degrading protease) from entomopathogenic fungus Beauveria bassiana(ATCC7159) in liquid medium by adding of gelatin, bovine serum albumin(BSA), casein and polypeptone. The optimal induction medium for production of extracellular proteases is composed of 0.5% polypeptone, trace elements and 50 mM potassium phosphate(pH 6.0). In this condition, the production of extracellular proteases increased rapidly after the 24hrs, peaking at the third day and there was little inductive effect in culture broth more than pH 7.0. The proteases were inhibited by phenyl methyl sulfonyl fluoride(PMSF). High activity of protease was showed both range of pH 8.5 and 11.5 and also detected by three different portions of slice gel derived from nondenaturing isoelectricfocusing gel. At least three different extracellular proteases are produced in optimal production medium when polypeptone is used as the sole carbon and nitrogen source.
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